ABSTRACT
Background: Wound infection is one of the frequent complications in patients with surgical operations. Staphylococcus aureus is the most common cause of surgical wound infections. Origanum vulgare, a common culinary herb, has been shown to have strong antimicrobial activity, especially against Gram-positive pathogens
Objective: This study was designed to investigate the antibacterial effects of O. vulgare on S. aureus in surgical wounds using a rat model
Methods: Twenty male Sprague-Dawley rats were divided randomly into two groups of treatment and control rats [1:1]. A circular incision was made on the dorsal inter-scapular region of each rat. Then, rats were inoculated topically with 1 × 104 CFU of S. aureus at the site of skin wounds. O. vulgare extract was applied to wounds twice a day during the experiment. Animals of the control group were left untreated
Results: The load of bacteria in untreated rats was 7 × 10[6] +/- 6 CFU/wound while this was 2 × 10[5] +/- 1 CFU/wound for the treatment animals, which was significantly lower
Conclusion: Results have showed that topical application of O. vulgare extract on the infected wounds included significant antibacterial activity against S. aureus
ABSTRACT
Wound infection has become a major medical problem in recent years. This is usually caused by Gram-positive bacteria, especially Staphylococcus aureus. Since antimicrobial resistance to current drugs has critically been developed in these causative microorganisms, substitution medicine has become one of the main interests within researchers. The aim of this study was to evaluate the healing activity of Origanum vulgare against surgical wounds infected by S. aureus. Twenty male Sprague-Dawley rats were randomly divided into two groups. Excisions were created surgically on the animals' skin and then infected with S. aureus. Group 1 was treated with an extract of O. vulgare while Group 2 was untreated. Wound biopsy specimens were collected on Days 5,10 and 16 and analyzed. Results showed that the hydroxyproline content in the treatment group was significantly higher in various post wounding days. The mean of hexosamine in the treated group was higher than in the control group. Protein content increased gradually in Day 10. Results of histopathological studies showed moderate to intense granulation tissue formation and neovascularization in the treated group on Day 10. Furthermore, the histopathological studies showed that intense matrix formation and collagen fiber deposition occurred in treatment group on Day 16 post wound, while intense granulation tissue formation was the prominent feature in control group. The present study has demonstrated that the ethanol extract of O. vulgare contains properties that accelerate wound healing activities compared to control group
ABSTRACT
In this study, the role of local injection of butyric acid [BA] with autogenous omental graft was evaluated in healing of experimental Achilles tendon injury in rabbits. Nine adult male New Zealand rabbits were anesthetized and a partial thickness tenotomy was created on both hindlimbs. In treated group, omental graft was secured in place using BA soaked polygalactin 910 suture. In control group, the graft was sutured without BA. Butyric acid and normal saline were injected daily to treatment and control groups for three days, respectively. Based on the findings, on day 15 after injury, the tendon sections showed that healing rate in BA treated group was higher than that in control group. Furthermore, at days 28 and 45, comparison between BA treated and control groups demonstrated that BA increased the healing rate but with no significance. In summary, results of this study show that application of BA with autogenous omental graft can improve healing process of damaged Achilles tendon
Subject(s)
Animals, Laboratory , Omentum , Transplantation, Autologous , Achilles Tendon/injuries , Rabbits , Wound HealingABSTRACT
Wound and its management are the most common health problems in human and veterinary medicine. Herbal therapy is becoming increasingly popular among patients and physicians. This study was conducted to evaluate the effects of 5% Urtica dioica ointment on full thickness wounds of nine cattle, were assessed by microscopic and macroscopic methods. After preparing the operating site, a total of two full thickness skin wounds [3×4 cm] were created on the both sides of neck of each cattle. The wounds were treated by Urtica dioica, and saline solution twice daily. On days 5, 11 and 21 specimens was collected from wounds and evaluated histologically. Our study showed significant difference between treated and control groups, in wound contraction and epithelization on days 21. In group treated with Urtica dioica ointment, the healing process was higher than from control group. There is not any different between treated and control groups on days 5 and 11 significantly. In conclusion, this study shows that the application of Urtica dioica increased the coetaneous wound healing in cattle